【摘要】 目的:探讨四氢叶酸还原酶(MTHFR)基因C677T多态性在子痫前期发病机制中的作用。方法:选择妊娠期高血压疾病组孕妇80例,其中妊娠期高血压组19例、轻度子痫前期组25例,重度子痫前期组36例;并随机选取同期的正常晚期妊娠者60例为正常对照组。分别用过柱法提取基因型DNA,并行聚合酶链反应物-直接测序法检测和分析MTHFR基因中C677T的分型。结果:妊娠期高血压疾病组中C677/T677基因型与正常对照组比较,差异有统计学意义(P<0.05),妊娠期高血压疾病组T677等位基因频率(48.8%)高于正常对照组(32.5%),两组比较,差异有统计学意义(P<0.05);正常对照组与轻度子痫前期组及重度子痫前期组的C677/T677基因型和T677等位基因频率比较,差异有统计学意义(P<0.05),与妊娠期高血压组比较差异无统计学意义(P>0.05)。结论:妊娠期高血压疾病患者的MTHFR基因C677T多态性可能是子痫前期发生的诱因之一;T677等位基因可能是子痫前期的易感基因。更多还原

【Abstract】 Objective:To investigate the role of polymorphism of MTHFR gene(C677T)in the pathogenesis of preeclampsia.Methods:80 cases with hypertensive disorder complicating pregnancy(HDP group)were selected in this study,in which including 19 gestational hypertention(group A),25 mild preeclampsia(group B),36 severve preeclampsia(group C).60 healthy late-pregnant women were selected randomly as control group.The genotype DNA was extracted by chromatographic column,and the typing of C677T in MTHFR gene was analysed by PCR-directly detection method.Results:There was obvious difference in C677/T677 genotype between HDP group and control group(P<0.05).T677 allele frequency was higher in HDP group than that in control group(0.488 VS 0.325,P<0.05);Furthermore,to C677/T677 genotype and T677 allele frequency,there was obvious difference between the control group and group B,group C(P<0.05).There was no difference between group A and control group in C677/T677 genotype and T677 allele frequency(P>0.05).Conclusions:Polymorphism of MTHFR C677T gene may induce preeclampsia and T667 is regarded as predisposing genes to preeclampsia.更多还原