【摘要】 研究了Rose bengal处理对菠菜类囊体膜及PSⅡ颗粒的叶绿素荧光发射光谱、蛋白质内源荧光发射光谱及DCIP光还原活性的影响.结果表明:单线态氧可改变类囊体膜的结构,并且可破坏PSⅡ反应中心及LHCⅡ中叶绿素分子的结合状态,引起类囊体膜PSⅡ天线系统中的叶绿素捕光效率下降,还可引起光合电子传递能力下降和类囊体膜蛋白构象的改变.更多还原

【Abstract】 Treat spinach thyiakoid membrane and PS Ⅱ particle with rose bengal （RB） in different concentration, which can generate ¹O₂ via illuminate by green light. Then the effects of O₂ on fluoresence emission spectra at room temperature, activity of DCIP photoreduction were studied. The results showed that ¹O₂ can change the protein conformation in thyiakoid membrane, destroy PS Ⅱ reaction centre and the combine of chlorophyll in light harvest complex Ⅱ （LHC Ⅱ）, so, reduce the light harvesting efficiency of LHC Ⅱ. This research also concluded that ¹O₂ can reduce the efficiency of electron transfer chain in thyiakoid membrane.更多还原