【摘要】 目的研究温州市瓯海地区宿主携带汉坦病毒的状况和型别。方法经间接免疫荧光(IFA)试验初筛为阳性的鼠肺标本,用巢式RT-PCR扩增阳性标本中汉坦病毒的S基因片段上的目的核苷酸序列(620~999nt),并以ClustalX(1.83)和Phylip3.63构建系统进化树进行分型和分子进化分析。结果温州市瓯海地区共捕获啮齿动物148只,5份鼠肺标本中汉坦病毒抗原阳性,其中褐家鼠3只,黄胸鼠2只,病毒携带率为3.38%,与汉坦病毒分型标准比较,均属于SEOV型。结论瓯海地区褐家鼠和黄胸鼠携带S1和S3亚型汉坦病毒,显示出汉坦病毒的多样性。更多还原

【Abstract】 Objective TO gain more insight into the staus of hantavirus(HV) infection and their genotype in rodents in Ouhai District of Wenzhou City of Zhejiang.Methods The partial S fragment(620-999nt) from the HV antigen positive rodents lungs detected by indirect immunofluoresence assay was amplified with nested RT-PCR using genetype-specific primers,the phylogenetic analysis was performed with ClustalX(1.83) and Phylip3.63 software.Results A total of 148 rodents were trapped in Ouhai District,HV antigen were identified in 5 out of these rodents lungs,3 of which from Rattus norvegicus and 2 from Rattus flavipectus,and the overall prevalence of HV in rodents was 3.38%.Conclusion The main genotypes of HV carried by Rattus norvegicus and Rattus flavipectus in Ouhai District are genotypes S1 and S3 SEOV,and the HV type is diverse in Ouhai District.更多还原