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饲料中三聚氰胺的ELISA检测方法的探讨

Discussion on Melamine Determination in Feed by ELISA Method

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【作者】 郭洁; 李宏; 贾青; 杨汉卿; 陈瑛; 冯西辉;

【Author】 GUO Jie1,LI Hong1,JIA Qing1,YANG Han-qing1,CHEN Ying2,FENG Xi-hui1(1.Feed Quality Supervision and Testing Center,Ministry of Agriculture,Xi’an 710016;2.Tongchuan City Yintai District Animal Husbandry Station,Shaanxi Tongchuan 727000,China)

【机构】 农业部饲料质量监督检验测试中心; 铜川市印台区畜牧站;

【摘要】 试验利用BRAX IS试剂盒和自制(经气相色谱质谱法确证不含三聚氰胺)三聚氰胺添加量为2 mg/kg的配合料和浓缩料作为阳性对照样品,对100批次饲料产品的进行了三聚氰胺筛选,并将两种方法的筛选结果按照NY/T1372-2007中的气相色谱质谱法进行了确证和比较。试验结果表明:利用三聚氰胺添加量为2 mg/kg配合料和浓缩料为阳性对照样品,对样品的筛选结果,要比使用BRAX IS试剂盒的内制曲线筛选的结果,产生的假阳性少,准确率相对要高。

【Abstract】 The research made use of BRAXIS kit and self-made compound and concentrated feed(via gas chromatography-mass spectrometry confirmed melamine-free) that melamine adding amount is 2 mg/kg as a positive control sample,screened melamine in 100 batches of pig and chicken feed products.Screening results of two methods in accordance were confirmed and compared with NY/T1372-2007 gas chromatography-mass spectrometry.The results showed that: the use of compound and concentrated feed that melamine adding amount is 2mg/kg as a positive control sample,the results were in fewer false-positive rate and higher accuracy than the use of BRAXIS Kit for the results of screening within the system of curves.

【关键词】 三聚氰胺; 检测; ELISA方法;
【Key words】 melamine; determination; ELISA method;
  • 【文献出处】 中国农业科技导报 ,Journal of Agricultural Science and Technology , 编辑部邮箱 ,2009年S1期
  • 【分类号】S816
  • 【下载频次】111
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