【摘要】 [目的]探讨三氧化二铁(Fe2O3)纳米颗粒对人肝癌细胞(HepG2细胞)形态学和游离钙([Ca2+]i)的影响,为研究Fe2O3纳米颗粒诱导细胞凋亡的机制提供依据。[方法]以不同浓度(0.173、0.347、0.694g/L)的Fe2O3纳米颗粒对HepG2细胞染毒1、12、24h,用激光扫描共聚焦显微镜检测HepG2细胞[Ca2+]i荧光强度的变化。[结果]与对照组相比,染毒不同时间各染毒组细胞内[Ca2+]i含量差异均有统计学意义(P<0.05);染毒不同浓度时,Fe2O3纳米颗粒均能不同程度地引起HepG2细胞的形态学改变及其细胞内[Ca2+]i的升高。0.347、0.694g/L组染毒不同时间点的平均荧光强度分别为127.33±20.13、108.88±19.15、144.00±13.86和119.67±1.15、122.35±11.47、186.40±17.34,均明显高于对照组54.33±6.43,差异有统计学意义(P<0.05)。[结论]HepG2细胞凋亡可能与Fe2O3纳米颗粒引起的细胞[Ca2+]i升高有关。更多还原

【Abstract】 [Objective] To study the effects of iron sesquioxide nanoparticles(nano-Fe2O3)on morphology and free calcium([Ca2+]i)concentration in human hepatoma cell line(HepG2 cell).To provide evidence for the mechanism of nano-Fe2O3-induced apoptosis in HepG2 cell.[Methods] HepG2 cells were exposed to nano-Fe2O3 of different concentrations(0.173,0.347,and 0.694 g/L)for 1,12,and 24 h.Laser scanning confocal microscopy was used to detect the fluorescence intensity of [Ca2+]i in HepG2 cells.[Results] Compared with the control group,treated for different time duration,differences of intracellular[Ca2+]i concentration between dose groups were significan(tP < 0.05).The morphology was changed and the[Ca2+]i concentration increased in HepG2 cell after exposure to different concentrations of nano-Fe2O3.Intracellular[Ca2+]i concentrations at 1,12,and 24 h exposure to 0.347,and 0.694 g/L nano-Fe2O3 were 127.33±20.13,108.88±19.15,144.00±13.86,and 119.67±1.15,122.35±11.47,186.40±17.34 respectively,and all higher than control group(54.33±6.43)(P < 0.05).[Conclution] HepG2 apoptosis might be related to the increase of the intracellular [Ca2+]i level caused by nano-Fe2O3.更多还原