【摘要】 在油茶cDNA文库和EST文库构建的基础上,根据油茶金属硫蛋白的EST序列设计特异引物,以油茶近成熟种子为材料,利用3’RACE技术,克隆到1个金属硫蛋白基因的cDNA全序列。该基因全长为675 bp,其中5’非编码区59 bp,3’非编码区379 bp,开放阅读框长234 bp,编码78个氨基酸;该蛋白含有14个半胱氨酸,分布在蛋白质的N端和C端,呈CC,CX,CXXC形式排列。预测该蛋白相对分子量为7 864.9 Da;等电点为4.86;37~48位氨基酸间有较强的疏水区。多序列比对发现油茶MT核苷酸序列与茶的相似性达到99%,推导的氨基酸序列与茶完全相同,因此将该基因命名co_m t1。更多还原

【Abstract】 A DNA library and a EST library of Camellia oleifera were constructed.According to the metallothionein’s EST sequences of Camellia oleifera,a specific primer was designed,the metallothionein gene was cloned from camellia oleifera with 3’RACE,respectively.This gene is 675 bp in length,including 58 bp of 5’untranslated region and 379 bp of 3’ untranslated region.It has an open reading frame of 234 bp,encoding a protein of 78 amino acid residues with protein molecular weight of 7 864.9 Da and isoelectric point of 4.86.The protein has 14 Cysresidues(Cys) and a conserved sequence from of C-C,C-X-C,C-X-X-C at the end of it.There is a strong hydrophobic area at 37-48 amino acid residues.The alignment of nucleotide acids sequence reveals that the comparability of Camellia oleifera and Camellia sinensis is 99%,and their amino acids sequence is similar.This gene is named co_mt1.更多还原