【摘要】 促进淋巴细胞分泌抗病毒干扰素和白介素等细胞因子是生物活性多糖抗病毒和增强免疫的机制之一。为探明sEPS5和sEPS2抗病毒和增强免疫作用的机理,用Primer Premier软件设计了1对白介素-2(Interleukin-2,IL-2)引物和1对干扰素-γ(Interferon-γ,IFN-γ)引物,用荧光定量PCR方法测定sEPS2和sEPS5对鸡外周血T淋巴细胞IL-2、IFN-γ的mRNA表达的影响,以未修饰的EPS为对照。结果表明,在62.50μg/ml和31.25μg/ml时,sEPS5和sEPS2对2种细胞因子的诱生作用显著强于未修饰的EPS,其中sEPS5的作用最强,sEPS2次之,并与剂量呈正相关。这可能是硫酸化多糖抗病毒和增强免疫的分子机制之一。更多还原

【Abstract】 To promote the secretion of interferon,interleukin and many other cytokines was one mechanism of the anti-virus and immune-enhancement actions of bioactive polysaccharides.In order to study the molecular mechanism of sEPS2 and sEPS5 in anti-virus and immune-enhancement actions,according to Primer Premier Software,two pairs of RT-PCR primers of IL-2 and interferon-γ(IFN-γ) were designed.The effects of sEPS5 and sEPS2 on the expression level of IL-2 and IFN-γ mRNA,isolated from ConA-stimulated peripheral blood T lymphocyte of chicken,were determined by fluorescence quantitative PCR assay,taking EPS as control.The results showed that sEPS2 and sEPS5 could improve the expression level of IL-2 and IFN-γ mRNA,and were significantly better than EPS at 62.5 μg/ml and 31.25 μg/ml.The action of sEPS5 was the strongest and in a dose-dependant manner.It might be one of the immunoenhancement mechanisms that polysaccharides could promote the secretion of IL-2 and IFN-γ.更多还原