【摘要】 目的评价腺病毒介导的人骨形态发生蛋白7(Ad-hBMP-7)基因转染的骨髓间充质干细胞(BMSCs)和纳米羟基磷灰石/胶原复合材料(NHAC)复合后对脊柱融合的影响。方法抽取大鼠骨髓获得BMSCs,应用Ad-hBMP-7转染大鼠BMSCs,将NHAC材料和密度为2×105/ml的Ad-BMP-7转染大鼠的BMSCs细胞孵育2h后备用;Wistar大鼠56只,随机分成4组,建立脊柱后外侧融合模型,分别植入自体髂骨(A组)、NHAC材料(B组)、NHAC材料和BMSCs(C组)以及Ad-hBMP-7-BMSCs与NHAC材料混合物(D组)。每组行RT-PCR及Western blotting检测,8周和12周后分别行X线摄片,并对标本进行手触检测硬度、生物力学检测和组织学观察。结果D组10只大鼠的脊柱L4/5节段全部融合;A组12周0例融合;B组12周0例融合;C组12周3例融合。D组同A、B、C组结果相比均有显著性差异(P<0.001)。结论BMP-7局部基因治疗和组织工程化骨能促进脊柱融合。更多还原

【Abstract】 Objective To evaluate effectiveness of ex vivo adenoviral gene transfer creating human bone morphogenetic protein 7-producing mesenchymal stem cells(Ad-hBMP-7 cells) compounded with nano-hydroxyapatite/collagen(NHAC) composite to induce spinal fusion in a rat posterolateral spine fusion model. Methods Mesenchymal stem cells were cultured and transduced by Ad-hBMP-7.Then Ad-hBMP-7 cells were cocultured with NHAC.Totally 56 Wistar rats were divided into four groups.Intertransverse spinal arthrodesis was attempted in four groups of Wistar rats with autogenous bone(group A);NHAC(group B);NHAC and BMSCs(group C);Ad-hBMP-7 cells and NHAC(group D).Each specimen underwent plain radiography,manual palpation and histological analysis. Results All spines in group D had fusion.In contrast,none of the spines in group A and group B had fusion.Three of ten rats in group C achieved fusion at the 12th week post-operatively.Conclusion BMP-7-producing mesenchymal stem cells compounded with NHAC composite may induce intertransverse fusion in the rat spine model.更多还原