【摘要】 目的探讨大鼠空间辨别性学习记忆活动与趋化因子基质细胞衍生因子-1(SDF-1)的关系。方法用免疫组织化学方法、免疫印迹法和RT-PCR方法对获得空间辨别性学习记忆大鼠海马结构内SDF-1表达进行研究,并与游水组和对照组比较。结果1.免疫组织化学方法显示,对照组大鼠海马结构内趋化因子SDF-1阳性细胞分布在海马结构各亚区和各层。阳性细胞的形态以圆形为主,少见锥体形和梭形。阳性产物主要分布在细胞膜上;游水组大鼠海马结构内SDF-1阳性细胞的形态和分布与对照组相比未见组间差异;训练组大鼠海马结构内SDF-1免疫阳性细胞的数量比对照组增多(P<0.01)。阳性细胞的形态以锥体形和三角形居多,在分布上有向齿状回聚集的趋势。2.免疫印迹方法检测训练组大鼠海马结构内SDF-1蛋白质的表达明显高于对照组(P<0.01),而且在训练组内则随着训练时间的延长表达量随之增加。3.RT-PCR方法检测训练组大鼠海马结构内SDF-1mRNA的量明显高于对照组(P<0.01),且随着训练时间的延长表达增加。在水盆中漫游的游水组大鼠,不论是免疫组织化学方法,还是免疫印迹法以及RT-PCR方法获得的结果与对照组相比均未见组间差异。结论经水迷宫训练使大鼠获得空间辨别性学习记忆功能,致使海马结构内细胞导向因子SDF-1表达上调,上调的SDF-1蛋白质可能与所获功能需要的形态基础-神经微环路的形成有关。更多还原

【Abstract】 Objective To explore the relation between spatial learning and memory and expression of SDF-1 chemokin in rat hippocampal formation. Methods Immunohistochemistry,Western-blotting and RT-PCR were used to study changes of SDF-1 protein in hippocampual formation during spatial learning and memory in rats. Results 1. The immunohistochemistry result showed that the SDF-1 immunoreactive positive neurons were mainly distributed in each subregion and each layer of hippocampus,and were mainly round in shape the while positive pyramidal and fusiform neurons were rare. The number of the positive neurons was more in trained group than that in control groups(P<0.01). 2. The Western blotting result showed that expression of SDF-1 protein was significantly higher in trained group than that in control groups(P<0.01). In trained group,expression of SDF-1 was gradually increased with training. 3. The RT-PCR result showed that level of SDF-1 mRNA transcription was significantly higher in trained group than that in control groups(P<0.01),and the level of SDF-1 mRNA in the trained group was gradually increased with training duration. There was no group differences between control group and swimming group as shown by immunochemistry,Western blotting and RT-PCR shown. Conclusion The spatial learning and memory induced expression of SDF-1 in rat hippocampus and it may have relation with formation of new microcircle pathway in hippocampus during the period of spatial learning and memory.更多还原