【摘要】 目的探讨溶血磷脂酸受体3(LPA-R3)mRNA和蛋白在小鼠胚胎着床过程中表达的动态变化。方法将78只雌性昆明小鼠随机分为真孕组(36只)、假孕组(36只)和对照组(6只),应用反转录-聚合酶链反应(RT-PCR)和Western blotting以及免疫组织化学SP法,检测胚胎围着床期(0~6d)LPA-R3 mRNA和蛋白在小鼠子宫内膜的表达和定位。结果LPA-R3主要分布于小鼠子宫内膜腔上皮、腺上皮和部分基质细胞的胞质。LPA-R3 mRNA和蛋白在小鼠胚胎着床过程中,3d时开始上升,4d时达峰值,5d、6d骤然下降至基础水平。假孕组子宫内膜LPA-R3mRNA和蛋白表达水平及变化规律与相应同期真孕组一致。结论LPA-R3可能参与了胚胎的着床和子宫内膜容受性的建立过程。更多还原

【Abstract】 Objective To investigate the expression and distribution of LPA-R3 during the period of mouse embryo implantation. Methods Seventy-eight mice were randomly divided into pregnancy group(n=36),pseudopregnancy group(n=36) and control group(n=6).Immunohistochemistry and reverse transcription polymerase chain reation (RT-PCR) and Western blotting were employed to analyze the expression and distribution of LPA-R3 in the uterine endometrium during mouse embryo implantation. Results LPA-R3 was mainly expressed in cytoplasm of glandular and luminal epithelium and part of stromal cells of mouse uterus.The LPA-R3 mRNA and protein level in the uteri increased during early pregnancy,increased at 3 days post coitus(3 d.p.c.),peaking around 4 d.p.c.,peri-implantation period,then returned to the basal levels on 5 d.p.c.This temporal profile of LPA-R3 expression in the uterus of pseudopregnant mice was essentially similar to that observed in normal pregnant mice.The expression level of LPA-R3 receptor protein and mRNA had no significant difference between pseudopregnant mice and normal pregnant mice(P>0.05).Conclusion LPA-R3 may participate in the period of mouse embryo implantation.更多还原