【摘要】 目的探讨雄激素对自身免疫性卵巢早衰的影响。方法50只小鼠随机分为空白组、早晚治疗组及对应模型组。治疗及模型组注射小鼠透明带多肽建立卵巢早衰模型。早晚治疗组分别于免疫第15、20天起腹腔注射丙酸睾酮共15 d。以阴道脱落细胞涂片、血抗透明带抗体浓度、卵巢中淋巴细胞浸润及成熟卵泡比例作为观察指标。结果对于性周期改变、血抗透明带抗体浓度、卵巢淋巴细胞浸润程度,模型组显著高于空白组,早治疗组显著低于模型组,早治疗组的血抗透明带抗体浓度显著低于晚治疗组。对于卵巢成熟卵泡比例,模型组显著低于空白组,早治疗组显著高于模型组及晚治疗组。结论雄激素能显著改善小鼠自身免疫性卵巢早衰,早期治疗效果明显优于晚期。更多还原

【Abstract】 Objective To identify the effects of androgen on mouse model of autoimmune ovary failure.MethodsFifty mice were randomly divided into five groups as control group,early therapeutic group and its model group,late therapeutic group and its model group.Autoimmune POF was induced in forty mice by Pzp3.Then,the animals were randomly divided into four groups: two were treated with androgen from the 15th and 20th day after immune.The control group was treated with distilled water.The therapeutic efficacy of each treatment was evaluated by the changes in sexual cycles of mice,the serum level of AzpAb,infiltration of cells positively expressing CD45 in the ovary as well as the percentage of growing follicles of the ovary.Results The sexual cycles,serum level of AzpAb,infiltration of CD45 cells in the ovary of each model group were more significant than those in control group;early therapeutic group was less than model group;and serum level of AzpAb in early therapeutic group was lower than that of late therapeutic group.The percentage of growing follicles in the ovary of each model group was lower than that of control group;early therapeutic group was higher than model group and late therapeutic group.Conclusion The use of androgen in mice with autoimmune POF may notably ameliorate the pathogenetic conditions of the disorder,and the treatment during prophase was apparently more effective than that during the advanced stage.更多还原