【摘要】 目的探讨FZS中药合剂对Aβ25-35、Aβ42、Aβ42寡聚体诱导的PC12细胞凋亡的保护作用。方法应用正常细胞进行对照研究,通过测定MTT、LDH和原位末端标记(TUNEL)方法检测细胞凋亡。原子力显微镜观察PC12细胞表面突起的变化。结果MTT在FZS组明显增高(P<0.05);LDH、TUNEL凋亡率在FZS组表达明显降低(P<0.05);原子力显微镜观察Aβ致细胞表面突起缩短的现象在FZS组明显改善,差异有显著意义(P<0.05)。结论FZS可以通过降低Aβ的细胞凋亡作用而减少其细胞毒性,FZS中药合剂可能成为Alzheimer病的治疗手段之一。更多还原

【Abstract】 Objective To investigate the protective effects of compound Chinese traditional medicine FZS to Aβ25-35、Aβ42 and Aβ42 oligomer induced apoptosis of PC12 cells.Methods Neuronal cells living were examined by MTT and LDH,while neuronal apoptosis examined by TdT-mediated dUTP-biotin nick end labeling(TUNEL) assays using a normal cell control testing.We have used atomic force microscopy(AFM) to visualize the PC12 cell surfaces.Results LDH and TUNEL were significantly decreased,while MTT was significantly increased in FZS group(P<0.05).The data showed that the surface projection of PC12 cell was shortened in Aβ group,and was significantly promoted with FZS treatment(P<0.05).Conclusion FZS may reduce toxicity of Aβ via reduction of apoptosis,which could be a novel strategy for Alzheimer’s disease(AD) therapeutics.更多还原