【摘要】 应用来源于粪产碱杆菌的青霉素G酰化酶催化合成头孢克洛,并对生物转化反应过程进行了研究。在反应条件:pH 7.0、25℃、磷酸钠缓冲体系、加酶量3 U/mL和底物浓度60 mmol/L时,转化率达到85%,表明粪产碱杆菌青霉素G酰化酶具有较高的催化活力。研究了新型分离纯化方法,经过树脂分离的产物纯度超过95%,主要杂质含量小于0.5%,可以达到药典要求,为生物法合成头孢抗生素走向产业化奠定了基础。更多还原

【Abstract】 Penicillin G acylase from Alcaligenes faecalis(AfPGA) was used for the biosynthesis of cephalosporins.Optimization of the reaction was accomplished and the optimal results were achieved in phosphate buffer of pH 7.0 at 25 ℃,with substrate concentration of 60 mmol/L and enzyme of 3 U/mL.Conversion of substrate reached 85%.The results proved that AfPGA was eximious on enzyme activity.(Isolation) method was studied and resin was applied for separation.The purity of product reached 95% and the main by-product was less than 0.5%.The results were according with the pharmacopoeia and made the biotransformation process possible for industrial application.更多还原