【摘要】 利用克雷伯氏杆菌以盾叶薯蓣糖化液为底物发酵生产2,3-丁二醇(2,3-BD),考察了2,3-BD浓度、生产强度、有机酸生成及代谢流量分布情况.结果表明,盾叶薯蓣中的有机酸成分能促进三羧酸循环途径和乙酸途径的代谢流,减弱琥珀酸途径的代谢流,从而提高2,3-BD的浓度.以盾叶薯蓣糖化液为底物,采用批式流加方式,补加固体葡萄糖,发酵56h,发酵液中2,3-BD最终浓度达到80.20g/L,乙偶姻与2,3-BD浓度之和最终达到86.19g/L,生产强度达到1.54g/(L-h),比单独以葡萄糖为底物时分别提高了8.50%,7.38%和7.69%.更多还原

【Abstract】 The fed-batch fermentation of 2,3-butanediol by Klebsiella pneumoniae from Dioscorea zingiberensis was investigated.The concentration and productivity of target products, organic acid metabolism and glucose metabolic flux distributions during the fermentation were analyzed.The results showed that organic acids in Dioscorea zingiberensis could promote metabolic flux in tricarboxylic acids cycle and acetic acid production pathway and decrease the butane diacid production pathway, thus, 2,3-butanediol production was increased.80.20 g/L of 2,3-butanediol, 86.19 g/L of 2,3-butanediol and acetoin, corresponding to 1.54 g/(L-h) of productivity, were obtained using the hydrolyzate of Dioscorea zingiberensis as substrate and feeding glucose during fed-batch fermentation, which increased 8.50%, 7.38% and 7.69%, respectively, compared with that using glucose as sole substrate.更多还原