【摘要】 [目的]探讨常氧下低氧诱导因子1α(HIF-1α)在传代培养LoVo细胞中的表达。[方法]在常氧下传代培养LoVo细胞,MTT法检测细胞增殖,绘制生长曲线,Western blot检测传代后不同时间点HIF-1α蛋白表达。[结果]细胞生长曲线示LoVo细胞在传代后24 h逐渐增加,72 h后迅速增加。Western blot示HIF-1α蛋白表达在传代后24 h最高,随时间延长,表达量逐渐降低。[结论]常氧下HIF-1α在LoVo细胞中可低水平表达,传代可引起HIF-1α表达增高,HIF-1α在促进细胞损伤的修复和细胞增殖中起重要作用。更多还原

【Abstract】 [Objective]To explore the expression of hypoxia inducible factor-1α(HIF-1α) under the condition of normoxia and passaged LoVo cells.[Methods]LoVo cells were cultivated and passaged under normoxia.MTT was used to detect cell proliferation and growth curve was made.Western blot was performed to analyze the protein expression of HIF-1α after passage at different time points.[Results]The number of LoVo cells was increasing at 24 h after passage and in a rapid way at 72 h after passage.The expression of HIF-1α protein was at the highest level at 24 h after passage,and was decreasing with the extension of time.[Conclusions]The expression of HIF-1α was at lower level under normoxia in LoVo cells,which was increased by passage.HIF-1α may play an important role in promoting damaged-cell repairmen and cell proliferation.更多还原