【摘要】 以1,3-二苯基异苯并呋喃(1,3-Diphenylisobenzofuran,DPBF)为荧光探针,研究了姜黄素(Curcumin,CUR)在铜离子催化下产生的单线态氧(1O2),其反应机理为姜黄素与溶液中的氧分子快速作用产生O2.-和H2O2等活性氧物种,同时Cu2+与姜黄素形成复合物,再与H2O2形成过氧化物过渡态,过氧化物进一步与H2O2发生类Haber-Weiss反应生成1O2,且只有Cu2+和Cu+离子可催化姜黄素并产生1O2.1O2在1.37×10-8~3.66×10-7mol/L浓度范围内与荧光强度下降值ΔIF有良好的线性关系,检出限为4.12×10-9mol/L.更多还原

【Abstract】 The production mechanism of singlet oxygen(1O2) in the curcumin catalyzed by copper ion was studied using 1,3-diphenylisobenzofuran(DPBF) as a selective fluorescence probe.The absorption spectrum of curcumin(CUR) binding to copper ion demonstrated the formation of copper ion-CUR complexes.This complexes were further oxidized by H2O2 giving rise to singlet oxygen,which was similar to Haber-Weiss reaction.That the metal ions catalyzed curcumin to produce 1O2 was tested.Except for Cu2+ and Cu+-CUR systems,no detectable 1O2 was produced in other metal ion-CUR systems.A new method was found for determining 1O2 in the curcumin system by catalysis of Cu2+.A linear relationship was obtained between ΔIF and the concentration of 1O2 in the range of 1.37×10-8—3.66×10-7 mol/L.The detection limit was 4.12×10-9 mol/L.更多还原