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棕笋中黄酮类物质的提取
Extraction and Determination of Flavones from Trachycarpus fortunei
【摘要】 研究了棕笋中黄酮类物质的提取工艺条件。在单因素试验的基础上,采用正交试验设计,以芦丁为对照品,采用紫外分光光度法测定棕笋中总黄酮的含量。得出最佳提取工艺条件:重复提取2次,硼砂浓度0.5%,温度70℃,料液比1∶12,提取40 min。为棕笋进一步研究和开发利用提供一定的科学依据。
【Abstract】 Extraction methods and determination of flavones from Trachycarpus fortunei were studied.Based on monofactorial tests,L9(3~4)orthogonal test was designed.And the total flavones of Trachycarpus fortunei were determined by UV-spectrophotometric method,using rutin as standard sample.The results showed that the best extraction condition was as following: 0.5%(M/V) Na2B4O7·10H2O as extraction agent,the ratio of material to liquid at 1∶12,40 minutes of extraction time,twice extraction,and extraction temperature at 70℃.The study provided scientific basis for further research,development and utilization of Trachycarpus fortunei.
- 【文献出处】 贵州农业科学 ,Guizhou Agricultural Sciences , 编辑部邮箱 ,2009年12期
- 【分类号】TQ461
- 【被引频次】3
- 【下载频次】154