【摘要】 本研究以小麦骨干亲本矮孟牛及其33个衍生品种(系)为材料,利用低分子量(LMW)麦谷蛋白Glu-B3位点的STS-PCR标记、醇溶蛋白Gli-B1位点的SSR标记和黑麦碱SEC-1b位点的STS-PCR标记进行复合PCR,检测1BL/1RS易位。结果表明,矮孟牛Ⅱ、Ⅳ、Ⅴ、Ⅵ和Ⅶ型含有1BL/1RS染色体,矮孟牛Ⅰ和Ⅲ型不含1BL/1RS;在矮孟牛的33个衍生后代中,25个含1BL/1RS,其余8个则不含1BL/1RS。利用A-PAGE技术对上述材料进行了黑麦碱蛋白的检测,结果与复合PCR一致,两种方法相结合能准确的检测1BL/1RS。更多还原

【Abstract】 Glu-B3 STS-PCR, Gli-B1 SSR and SEC-1b STS-PCR markers were used to identify 1BL/1RS translocation lines in Aimengniu and its derivatives. The results indicated that Aimengniu Ⅱ, Aimengniu Ⅳ, Aimengniu Ⅴ, Aimengniu Ⅵ and Aimengniu Ⅶ were 1BL/1RS translocation lines, whereas AimengniuⅠ and Aimengniu Ⅲ were not 1BL/1RS translocation lines. Among the 33 derivatives, there were 25 carrying 1BL/1RS translocation lines, while the others not. Secalins were detected by acid polyacrylamide gel electrophore- sis (A-PAGE), and the results were accordant with that of multiplex PCR, so the combination of the two meth- ods can test the 1BL/1RS translocation lines correctly.更多还原