【摘要】 将离子液体、分散液相微萃取与超声萃取技术结合,采用疏水性离子液体1-丁基-3-甲基咪唑六氟磷酸盐([C4 MIM][PF6])为萃取剂,建立了超声辅助离子液体分散液相微萃取-高效液相色谱法分析废水中3种雌激素物质(己烯雌酚、双烯雌酚、己烷雌酚)方法.试验采用50μL的离子液体,考察了溶液体积、溶液pH值、超声时间、静置时间、离心时间等因素对富集效果的影响.最佳的萃取条件为:溶液体积为6 mL,甲醇体积0.3 mL,溶液pH值为2.0,超声时间6min,静置时间30min,离心时间10 min.在优化的萃取条件下,3种雌激素的富集倍数可达到96.8~112.4倍;方法的线性范围为0.5-100.0μg/L;检出限为0.25~0.50μg/L.对浓度为5.0μg/L的3种物质测定6次的相对标准偏差为9.2%~10.8%.更多还原

【Abstract】 A novel method was established for the determination of estrogens in wastewater by ultrasonic assisted ionic liquid(1-butyl-3-methylimidazolium hexafluorophosphate,[C4MIM] \)dispersive liquid-phase microextraction coupled with high performance liquid chromatography.Some conditions that might affect the extraction efficiency,such as volume of solution,pH value of solution,ultrasonic time,hierarchical standing time,and centrifuging time were optimized.The results indicated that the optimal conditions were: 6 mL of solution at pH 2.0 for 6 min of ultrasonication,30 min of hierarchical standing and 10min of centrifugation.Under the above mentioned conditions,the extraction efficiency of ionic liquid based ultrasonic microextraction for the three estrogens were in the range of 96.8～112.4.Their linear ranges and detection limits were 0.5-100 μg/L and 0.25～0.50 μg/L respectively.The proposed method was applied to determine three estrogens in wastewater.The recoveries of three estrogens spiked in wastewater were in the range of 89.0%～96.6%.更多还原