【摘要】 目的探讨TRAIL与化疗药物5氟尿嘧啶(5-FU)单独和联合对喉癌HEP-2细胞株增殖、凋亡的影响并探讨其可能机制。方法不同浓度的TRAIL、5-FU单独及联合作用于HEP-2细胞株,MTT法检测其增殖抑制作用,FCM法检测细胞凋亡率,荧光显微镜下观察细胞形态,Western blot法检测c-FLIP、NF-κB蛋白的表达。结果TRAIL与5-FU联合作用于HEP-2细胞株时,其抑制率、凋亡率较单独作用时高,c-FLIP、NF-κB蛋白的表达水平低于单独作用时的表达水平。结论TRAIL与5-Fu联合作用有协同诱导HEP-2细胞株凋亡的作用,可能机制为下调c-FLIP、NF-κB蛋白的表达水平。更多还原

【Abstract】 ObjectiveTo observe the effects TRAIL,5-FU and their combination on the proliferation and apoptosis of HEP-2 cell line,and to explore the possible mechanism.MethodsTRAIL(10.0 ng/ml) and 5-FU(1.0 μg/ml) were used.HEP-2 cells were divided into control group,TRAIL treated group,5-FU treated group,5-FU+TRAIL treated group.The inhibition on the proliferation of HEP-2 cells respectively treated with TRAIL,5-FU and their combination was detected by MTT and their apoptosis rate was detected by FCM.Cell morphology was observed by fluorescence microscopy.The protein expressions of c-FLIP and NF-κB in all groups were detected by Western blotting.ResultsThe inhibition rate and apoptosis rate of 5-FU+TRAIL treated cells was significantly higher than those of TRAIL treated cells and those of 5-FU treated cells.The expressions of c-FLIP and NF-κB in TRAIL treated cells and 5-FU treated cells were higher than those in TRAIL+5-FU treated cells.ConclusionTRAIL alone or combined with 5-FU can induce the inhibition and apoptosis of HEP-2 cell line,and the mechanism may be through restraining the protein expressions of c-FLIP and NF-κB.更多还原