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丛枝菌根(AM)真菌对翅果油树幼苗根系的影响

Effects of Arbuscular Mycorrhizal Fungi on the Seedling Roots of Elaeagnus mollis Diels

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【作者】 袁丽环闫桂琴朱志敏

【Author】 YUAN Li-huan,YAN Gui-qin,ZHU Zhi-min(Institute of Biodiversity,Shanxi Normal University,Linfen 041000,China)

【机构】 山西师范大学生物多样性研究所

【摘要】 采用接种和不接种(对照)丛枝菌根的方法对翅果油树幼苗在不同生长时期的形态、生物量、矿质元素含量及抗逆性等指标进行比较分析,以探讨丛枝菌根(AM)改善翅果油树在生态系统中的退化及濒危现状的机理.结果显示:(1)摩西球囊霉(Glomus mosseae)和脆无梗囊霉(Acaulospora delicata)与盆栽翅果油树苗木可形成菌根;菌根化苗木的形态及生物量等指标均与对照差异极显著,且与菌根侵染率呈显著正相关(P<0.05).(2)菌根使苗木根系体积增大、总吸收面积增加、根系的活跃吸收面积显著增加,且根系中活跃吸收面积比与磷、钾元素含量呈显著正相关(P<0.05).(3)苗木生长的盛期和末期,菌根化苗木根系过氧化物酶活性显著高于对照,而在生长后期却显著低于对照呈负相关(P<0.05);菌根化苗木根系多酚氧化酶活性显著高于对照(P<0.05),在苗木生长盛期酶活性最强,但接种不同菌种的苗木间差异不显著.研究表明,与不接种相比,接种菌根增加了翅果油树的根系吸收能力,提高了根系酶体系,有利于植物抵抗各种胁迫,对扩大翅果油树植物的分布区有重要意义.

【Abstract】 In order to explore the mechanism of the arbuscular mycorrhizal(AM) to improve Elaeagnus mollis at the degradation of ecosystems and endangered status,we study E.mollis seedlings vaccinated and non-arbuscular mycorrhizal inoculation at different growth patterns during the period,biomass,mineral element content and resistance.The results show that mycorrhizals form between Glomus mosseae,Acaulospora delicata and potted seedlings of E.mollis.Mycorrhizal seedling morphology and biomass and other indicators showed significantly positive correlation(P<0.05) with the mycorrhizal infection rate.It is demonstrated that E.mollis is a dependent tree species on mycorrhiza.The mycorrhiza fungi make the volume of root area and the total absorption increase,especially the obvious increase of the roots active absorption area of the seedlings.Moreover,the proportion of active absorption area in the roots presents the markedly positive correlation with the contents of the elements of potassium and phosphorus(P<0.05).During the peak and end period of seedling growth,the activity of peroxidase in the roots of mycorrhizal seedlings is higher than that of control,lower than that of controls in the late period(P<0.05).The activity of polyphenol oxidase in the mycorrhizal seedling roots is higher than that of the control,and attain the maximium in the peak period of seedling growth.The difference between the seedlings inoculated with different mycorrhiza fungi is not significant.The results indicated that E.mollis seedlings inoculated with mycorrhiza fungi can increase root absorption capacity,improve the root enzyme system and is conducive to a variety of plant resistance to stress.The expansion of Elaeagnus mollis plant distribution area has important significance.

【基金】 山西省自然科学基金项目(NO2008011058-1)
  • 【文献出处】 西北植物学报 ,Acta Botanica Boreali-Occidentalia Sinica , 编辑部邮箱 ,2009年03期
  • 【分类号】S792.99
  • 【被引频次】39
  • 【下载频次】449
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