【摘要】 以4龄金鳟Oncorhynchus mykiss为试验鱼(雄、雌性平均体重分别为1.7、2.2 kg),采用人工诱导雌核发育技术灭活金鳟精子,对受精卵进行热休克处理后正常孵化,获得初孵仔鱼;再用雄性激素诱导全雌二倍体,得到性逆转雄鱼(伪雄鱼);培育至性成熟后进行人工繁殖,采用热休克法对受精卵进行处理获得全雌三倍体鱼类。结果表明:金鳟全雌二倍体的诱导出现率为80%,金鳟伪雄鱼获取成功率为75%;用不同pH人工精浆激活伪雄鱼精巢精子,高pH(9~10)的人工精浆激活受精卵的效果最好,金鳟三倍体的出现率为75%~80%,其中还有一定比率的嵌合体出现。更多还原

【Abstract】 Gynogenesis techniques were artificially used to treat the ova in 4-year old golden rainbow trout(Oncorhynchus mykiss) weighing 1.7 kg for males and 2.4 kg for females and newly hatched larvae were obtained by induction of all-female diploid to get the all-female triploid,which were obtained from the fertilized eggs treated by heat shock,to sex reversed males(Pseudo-milter) by androgens with 80% of successful rate.The inversion rate of pseudo-milter was found to be 75%.The fact that the spermatozoa were artificially activated in seminal plasma with different pH values revealed that 75%-80% of the triploid frequency with some segmental chimera was observed in the artificial seminal plasma with pH value as high as 9-10.更多还原