【摘要】 大豆花叶病毒病（soybean mosaic virus,SMV）是世界性大豆病害,严重危害大豆的产量和质量。SC-11为我国黄淮夏大豆区以及北方春大豆区SMV主要流行株系。研究大豆品种对SC-11的抗性遗传方式,不同大豆材料对SC-11抗性位点的等位性关系,并对抗性基因进行了SSR标记定位。结果表明:齐黄1号对SC-11的抗性由一对显性基因（RSC-11）控制;齐黄1号、齐黄22、广吉和早熟18对SC-11的抗病基因是等位的或紧密连锁的;经分离群体组群分析法研究发现,齐黄1号抗SC-11的位点RSC-11位于F连锁群,与SSR标记Satt114、Satt334、Sat₂34和Sct₀33紧密连锁,距离分别为11.1 cM、8.9 cM、4.6 cM和4.7 cM;选取F连锁群上亲本间有多态的18对引物构建了F连锁群的遗传图谱,全长254.8 cM,标记间平均距离为13.41 cM。研究结果为大豆抗病育种以及抗性基因的精细定位和图位克隆奠定了基础。更多还原

【Abstract】 Soybean mosaic virus（SMV）is one of major viral diseases in soybean production worldwide.It causes yield loss and seed quality deficiency seriously.SMV strain SC-11 is a common prevalent strain in both of the Huang-Huai summer planting soybean region and the northern China spring planting soybean region.The major objectives of this research were to determine the inheritance of resistance,test the allelism of the resistance genes among the resistant cultivars,map the resistant gene to SC-11.The results showed that Qihuang No.1 has a single dominant resistance gene to SC-11.The resistance genes to SC-11 among Qihuang No.1,Qihuang 22,Kwanggyo and Zaoshu 18 were allelic.Bulk segregant analysis（BSA）demonstrated that in the F2 mapping population from Qihuang No.1×Nannong1138-2,Four SSR markers Satt114,Satt334,Sat₂34 and Sct₀33 in F linkage group were found closely linked to RSC-11,with genetic distances of 11.1cM,8.9 cM,4.6 cM and 4.7 cM,respectively.RSC-11 was located between Sat₂34 and Sct₀33 in F linkage group.Using 18 SSR markers locating in F linkage group of the integrated joint map and expressing polymorphism between the two parents,the genetic map of the F linkage group was constructed.The full length of the linkage group is 245.8cM with an average interval of 13.41cM between two markers.更多还原