【摘要】 以小叶杜鹃新生嫩芽为外植体,应用均匀设计法筛选其最适合的嫩芽基部直接再生芽苗、生根及种质试管保存的培养基,研究结果表明,最适合的基部直接再生芽苗诱导培养基为:DR+反式ZT3.00mg·L-1,诱导率达96%以上;生根培养基:MS(改良)+IAA0.50mg·L-1+IBA0.10mg·L-1+KT0.10mg·L-1,生根率达98.5%以上;试管保存培养基:N-68+B92.30mg·L-1+根皮苷1.50mg·L-1,保存时间可达32个月以上。以再生植株的茎节为材料进行快繁的结果表明,在40d的1个培养周期内,增殖倍数平均达70以上。常温条件下,采取"矮化延缓生长"的方法在试管内保存种质资源。成功建立了小叶杜鹃的离体培养和种质试管保存体系。更多还原

【Abstract】 The most suitable culture media for shoot regeneration at the base of tender buds,rooting and germplasm conservation in vitro were screened out by uniform design using tender buds of Rhododendron parvifolium Adams. as explants. Results showed that DR culture medium supplemented with 3.00mg·L-1 trans-ZT was suitable for shoot regeneration,with a regeneration rate of more than 96 percent; the modified MS medium with 0.50mg·L-1 IAA,0.10mg·L-1 IBA,and 0.10mg·L-1 KT for rooting,with a rooting rate of more than 98.5 percent; N-68 with 2.30mg·L-1 B9 and 1.50mg·L-1 phloridzin for germplasm conservation in vitro for 32 months. Stems,each with one node,were cut from the regenerated shoots and cultured for propagation,and a 70-fold of proliferation rate was achieved within 40 days. The method of deferring growth by stunting was applied to in vitro germplasm conservation at normal temperatures. In vitro culture and germplasm conservation system of R. parvifolium were successfully established.更多还原