【摘要】 目的:通过蛋白质组技术阐明羊布鲁氏菌外膜蛋白的主要组成。方法:提取并纯化羊布鲁氏菌的外膜蛋白,应用双向电泳技术进行分离,选取双向电泳图上主要的蛋白点进行质谱分析,同时主要蛋白质点的肽指纹图谱利用Mascot在NCBInr蛋白数据库中进行检索。结果:共鉴定到67个蛋白点,主要外膜蛋白为Omp25和Omp31,其他外膜蛋白如Imp、Frp、GroEL等,功能涉及物质运输、能量代谢、应激等。结论:对主要外膜蛋白Omp25和Omp31的识别与分析不仅有助于对布鲁氏菌致病机制的研究,而且为布鲁氏菌病的疫苗研制提供靶标蛋白。更多还原

【Abstract】 Objective To identify the major outer membrane proteins(OMPs) of Brucella Melitensis with proteome analysis.Methods The OMPs were extracted from the culture of Brucella Melitensis and then the two dimension electrophoresis was used to separate the OMPs.By means of matrix-assisted laser descorption/ionization time of light mass spectrometry(MALDI-TOF-MS),the OMPs were identified.Peptide mass fingerprints were searched in the NCBInr database using the Program Mascot.Results Sixty seven protein spots were identified,in which Omp25 and Omp31 were identified as two main OMPs and other OMPs,such as Imp,Frp and GroEL were found.The functions of identified OMPs included thymidylate kinase,protein precursor,NADH dehydrogenase subunit,cytosol aminopeptidase,etc.Conclusion Identification and analysis of Omp25 and Omp31 provides an important clue for understanding the molecular mechanism of Brucella Melitensis and developing the vaccine to prevent Brucella attack.更多还原