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转化用抗生素对青花菜离体再生的影响

Effect of Antibiotics for Transformation on in vitro Regeneration of Broccoli

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【作者】 秦耀国； 曹必好； 杨翠芹； 陈国菊； 雷建军；

【Author】 QIN Yao-guo1,2,CAO Bi-hao2,YANG Cui-qin3,CHEN Guo-ju2,LEI Jian-jun2(1.College of Forestry and Horticulture,Sichuan Agricultural University,Ya,an,Sichuan 625014,China;2.College of Horticulture,South China Agricultural University,Guangzhou,Guangdong 510642,China;3.College of Agriculture,Sichuan Agricultural University,Ya,an,Sichuan 625014,China)

【机构】 四川农业大学林学园艺学院； 华南农业大学园艺学院； 四川农业大学农学院；

【摘要】 为确定农杆菌转化青花菜时所用抗生素的适宜浓度,在分化与生根培养基中分别添加不同浓度的卡那霉素(Km)5、00 mg/L的羧苄青霉素(Cb)与头孢霉素(Cef),探讨了对青花菜离体再生的影响。结果表明:青花菜外植体对Km比较敏感,表现在分化阶段10 mg/L Km能抑制带柄子叶与下胚轴分化不定芽,在生根阶段15 mg/L Km能完全抑制根的生出。在脱菌抗生素的使用上,分化阶段宜用Cb,并逐步降低浓度,生根阶段宜用低浓度的Cef。

【Abstract】 To determine the appropriate concentration of antibiotics in Agrobacterium transformation of broccoli,different concentration of kanamycin,500 mg/L Cb and Cef were added into differentiation and rooting medium to discuss their effects on in vitro regeneration of broccoli.The results showed that explants of broccoli were more sensitive to kanamycin.10 mg/L kanamycin could inhibit cotyledons with petiole and hypocotyls from differentiating adventitious buds,and 15 mg/L kanamycin was able to inhibit roots from taking entirely.As regards the use of antibacterial antibiotics,carbenicillin was apt to be used in the differentiation stage and the concentration should be reduced gradually.Low concentration of cefotaxine was apt to be used in rooting stage.

【关键词】 青花菜； 再生； 分化； 抗生素；
【Key words】 Broccoli； Regeneration； Differentiation； Antibiotics；
【基金】 广东省重大专项资助项目(2002A2070301)
  • 【文献出处】 北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2009年03期
  • 【分类号】S635.3
  • 【被引频次】2
  • 【下载频次】74
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