【摘要】 目的本实验在细胞水平研究异丙肾上腺素(ISO)对脂多糖(LPS)诱导的SD大鼠肺泡巨噬细胞(AM)分泌TNF-α及IL-10的影响。方法分离SD大鼠肺泡巨噬细胞,调节浓度为1×106。分组如下:①空白组,②LPS(10μg/ml)组,③ISO组,④LPS+ISO组。以异丙肾上腺素(浓度分别为10μmol/L、100μmol/L、300μmol/L)加入SD大鼠肺泡巨噬细胞培养液。2h后,用脂多糖(10μg/ml)刺激,孵育6h后,以酶联免疫法测定上清中TNF-α,24h后,测定上清中IL-10的含量。结果与对照组相比,ISO组没有明显变化,LPS组TNF-α及IL-10的活性显著增加,10μmol/L、100μmol/L、300μmol/LISO均能显著降低LPS诱导的大鼠肺泡巨噬细胞释放TNF-α,但是能促进LPS诱导的大鼠肺泡巨噬细胞释放IL-10,且呈剂量依赖性,并且能增加巨噬细胞对中性红的吞噬能力。结论异丙肾上腺素能调节巨噬细胞分泌功能,使SD大鼠肺泡巨噬细胞减少炎性因子TNF-α分泌,增加抑炎因子IL-10分泌.增强巨噬细胞的吞噬中性红的能力。更多还原

【Abstract】 Objective This study was to observe the effect of Isoprenaline on lipopolysacchride (LPS)-induced secreting of TNF-α and IL-10 in Alveolar Macrophages (AM) of SD rats. Methods AM were isolated from normal rats and adjusted to 1×106 / ml and incu- bated in the following groups: ①Control group; ②L PS group; ③ISO group:10μM, 100μM, and 300μM in respectively;④LPS+ ISO group: L PS 10μg/ ml; ISO: 10μM, 100μM, and 300μM. Isoprenaline at various concentrations (10 μmol/l, 100 μmol/l, 300 μmol/l) were added in AM cells culture fluid, then stimulated with 10ug/ml LPS after 2 hours, the supernatants were collected for measuring TNF-α and IL-10 by ELISA kit after 6h and 24 h respectively. Resealts Compared with Control group, ISO group has no change, but 10ug/ml LPS significantly induced the production of TNF-α and IL-10, When Isoprenaline in combination with LPS was added into the medium, the TNF-α production was suppressed and the IL-10 production was increased in a dose-dependent manner Isoprenaline could increase the capability of phagocytizing neutral red. Conclusion Isoprenaline down-regulates LPS-induced pro-inflammatory mediators production in macrophage, but up-regulates LPS-induced anti-inflammatory mediators production. Isoprenaline increases the capability of phagocytizing neutral red.更多还原