【摘要】 [目的]为林蛙种属的科学划分提供依据。[方法]以中国林娃和黑龙江林娃为材料,采用聚丙烯酰胺凝胶电泳法对其血清蛋白进行比较分析。[结果]从中国林蛙血清中共分离出25条蛋白带,从黑龙江林娃血清中共分离出23条蛋白带,其中迁移率为0.552、0.524、0.400、0.375、0.331、0.241、0.076、0.062、0.048的9条蛋白带为中国林蛙特有;迁移率为0.517、0.269、0.228、0.214、0.127、0.069、0.041的7条蛋白带为黑龙江林娃特有,迁移率为0.621、0.469、0.434、0.359、0.317、0.297、0.200、0.172、0.159、0.145、0.124、0.103、0.090、0.055、0.028、0.014的16条蛋白带为两者共有。[结论]中国林娃与黑龙江林娃应归于2个独立的蛙种。更多还原

【Abstract】 [Objective] The study was to provide the basis for the scientific classification of rana species.[Method] With Rana chensinensis and Rana amurensis as the materials,their serum protein was comparatively analyzed by polyacrylamide gel electrophoresis method.[Result] 25 protein bands were separated from R.chensinensis serum and 23 protein bands were separated from R.amurensis serum.9 protein bands which mobility were 0.552,0.524,0.400,0.375,0.331,0.241,0.076,0.062,0.048 resp.were the particular bands of R.chensinensis and 7 protein bands which mobility were 0.517,0.269,0.228,0.214,0.127,0.069,0.041 resp.were the particular bands of R.amurensis.16 protein bands which mobility were 0.621,0.469,0.434,0.359,0.317,0.297,0.200,0.172,0.159,0.145,0.124,0.103,0.090,0.055,0.028,0.014 resp.were the mutual bands of R.chensinensis and R.amurensis.[Conclusion] R.chensinensis and R.amurensis should belong to 2 independent rana species.更多还原