【摘要】 [目的]为杂种小麦加工品质预测和优质小麦亲本组配提供依据。[方法]以杂交小麦母本晋麦47和父本丰优1号及其F2籽粒为材料,采用SDS-PAGE电泳法对小麦籽粒中的高分子量麦谷蛋白亚基(HMW-GS)的分离及表达进行分析。[结果]F2籽粒中共出现21种HMW-GS带型组合,其中回归母本的带型(N、7+9、3+12)占69.10%,回归父本的带型(1、17+18、5+10)占1.04%,其余29.86%为双亲杂合带型或缺失带型;HMW-GS在F2籽粒中按一定规律进行分离,G lu-D lb,G lu-D ld出现基因重组(重组率5.90%),17+18和7+9亚基同时在G lu-B1位点表达,而G lu-B lc在该位点出现表达空位(空位率5.20%)。[结论]明确了HMW-GS在小麦F2籽粒中的表达及分离规律。更多还原

【Abstract】 [Objective] The aim was to provide the basis for forecasting processing quality of hybrid wheat and combining parents in high quality wheat breeding process.[Method] With grains of female parent Jinmai 47,male parent Fengyou 1 and F2 of hybrid wheat as the materials,the isolation and expression of high molecular weight glutenin subunits(HMW-GS) in grains were analyzed by SDS-PAGE electrophoresis.[Result] 21 kinds of band phenotypes of HMW-GS appeared in F2 grains.Among which band phenotypes that regressed to female parent(N,7+9,3+12) was 69.10%,and that regressed to male parent(1、17+18、5+10) was 1.04%,the remained 29.86% band phenotypes were hybrid band phenotypes and deletion band phenotypes..HMW-GS isolated in F2 grains according to a certain inheritance,Glu-Dlb and Glu-Dld was recombined(recombination rate of 5.90%),17+18 and 7+9 subunits expressed at Glu-B1 site at the same time,while there were no expression of Glu-Blc at this site(vacancy rate of 5.20%).[Conclusion] This study determined the law of expression and isolation of HMW-GS in F2 grain of wheat.更多还原