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几种实验动物垫料的物理特性比较
Comparison between the Physical Characteristics of Several Laboratory Animals Bedding Materials
【摘要】 [目的]比较研究刨花、玉米棒芯、蒲草叶垫料的吸水、吸氨及保暖等性能,为开发新型优质实验动物垫料提供科学依据。[方法]以24 h吸水量为衡量垫料吸水能力的标准,对Co60-γ辐射灭菌、高压蒸汽灭菌、干热灭菌和不做灭菌处理的垫料进行吸水性试验;用GS-3型大气采样机采集笼内及房舍内氨气,用纳氏比色法在557型分光光度计测定并计算所采样品的氨浓度;用半导体温度计测定小鼠巢温及离巢后空巢余温。[结果]蒲草垫料的吸水能力高于刨花垫料(P<0.05)和玉米棒芯垫料(P<0.01),且刨花垫料的吸水能力高于玉米棒芯垫料(P<0.05)。小鼠饲养换笼后第4天及第7天笼内氨浓度,刨花垫料高于蒲草垫料(P>0.05)、低于玉米棒芯垫料(P<0.05),但蒲草垫料显著低于玉米棒芯垫料(P<0.01)。在(14.4±2.1)℃室温下,小鼠离巢后5、10、15 m in巢内余温,蒲草垫料均高于刨花垫料(P>0.05)及玉米棒芯垫料(P<0.05)。[结论]蒲草垫料是一种应用前景较好的新型垫料。
【Abstract】 [Objective] In order to get scientific basis for developing new-type and high-quality bedding materials for laboratory animals,the characteristics of Absorbing water,sucking ammonia air,and keeping warm of bedding materials(shaving,corncob and herb) was investigated.[Method] 24 hours’ water Absorption weight was taken as the standard of bedding materials’ water Absorption capacity;the water Absorption test was conducted on the bedding materials with Co60-γ radiation sterilization,high pressure steam sterilization,dry heat sterilization and without sterilization treatment respectively;GS-3 atmosphere sample collector was used to collect the ammonia gas in the cages and houses;ammonia sample concentration was determined by Nessler method on the Spectrophotometer 557 model;semiconductor thermometer was used to determine rats cages temperature and residual temperature.[Result] The results showed that the water Absorption capacity of herb was higher than shaving’s(P<0.05) and corncob’s(P<0.01),and the water Absorption capacity of shaving was higher than corncob’s(P<0.05).The ammonia density of bedding material was measured in fourth and seventh day after rats were raised and changed to other cages.The ammonia density of shaving was higher than herb’s(P>0.05) and lower than corncob’s(P<0.05),but that of herb’s was obviously lower than corncob’s.At room temperature(14.4±2.1) ℃,the residual heat of cages was measured in 5,10 and 15 min after rats had left.The residual heat of herb was higher than shaving’s(P>0.05) and corncob’s(P<0.05).[Conclusion] Herb is a sort of new-type bedding materials with quite good application foreground.
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2009年02期
- 【分类号】S865.1
- 【被引频次】13
- 【下载频次】310