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半胱氨酸合成酶基因CPCSaseA植物表达载体的构建
Construction of the Plant Expression Vector of the Cysteine synthase gene CPCSaseA
【摘要】 半胱氨酸合成酶是植物硫代谢过程中催化O-乙酰丝氨酸和硫化氢合成半胱氨酸的关键酶。用全长cDNA文库EST随机测序的方法获得了蜡梅的半胱氨酸合成酶胞质异形体基因,构建了全长正义植物表达载体pB I121-CPOASTL。
【Abstract】 Cysteine synthase(CSase),a key enzyme of plant sulfur metabolism,catalyzes the formation of Cys from sulfide and O-acetylserine.In this paper,the gene of cytosolic isoform of Cysteine synthase was isolated from Chimonanthus praecox,adopting the method of randomly sequencing the ESTs of full-length cDNA library,Constructed a full-length and sense plant expression vector pBI121-CPOASTL,Laid a solid foundation for further work.
【关键词】 半胱氨酸合成酶;
CPCSaseA基因;
植物表达载体;
【Key words】 Cysteine synthase; CPCSaseA; plant expression vector;
【Key words】 Cysteine synthase; CPCSaseA; plant expression vector;
【基金】 重庆市科委自然科学基金项目CSTC,2007BB1252
- 【文献出处】 安徽农学通报(上半月刊) ,Anhui Agricultural Science Bulletin , 编辑部邮箱 ,2009年13期
- 【分类号】Q943.2
- 【被引频次】1
- 【下载频次】139