【摘要】 背景与目的:探讨大肠癌患者循环肿瘤细胞p53基因的表达和端粒酶活性的变化及其临床病理学意义。材料与方法:113例大肠癌患者、13例良性大肠肿瘤患者术前的外周血和28例健康供血员的外周血,用分层液分离含有循环肿瘤细胞的单个核细胞,应用流式细胞仪和TRAP-ELISA法,分别检测p53基因表达和端粒酶活性。结果:在105例有信息的大肠癌患者中,p53基因表达和端粒酶活性的阳性率分别为74.3%(78/105)和61.0%(64/105)。异常的p53基因表达和端粒酶活性水平均与大肠癌患者的淋巴结转移显著相关(P<0.05),两者的联合检测提高了对大肠癌淋巴结转移的预测水平。p53基因异常表达还与大肠癌细胞分化程度显著相关(P=0.003)。结论:循环肿瘤细胞异常的p53基因表达和端粒酶活性是大肠癌的恶性生物学标志,两者密切相关,联合检测可提高对淋巴结转移状态的预测水平。更多还原

【Abstract】 BACKGROUND AND AIM:To study the clinicopathological significance of p53 gene expression and telomerase activity in circulating tumor cells of patients with colorectal carcinoma.MATERIALS AND METHODS:Mononuclear cells containing circulating tumor cells were isolated by Ficoll-Hypaque gradient centrifugation in peripheral blood from 113 preoperative patients with colorecatal carcinomas,13 patients with benign colorectal disease,and 28 healthy volunteers.FCM and TRAP-ELASA were used to evaluate p53 gene expression and telomerase activity,respectively.Experimental data were analyzed by SPSS 11.0 software and χ2 test.RESULTS:Among 105 informative patients with colorectal carcinoma,positive rate of p53 expression and telomerse activity were 74.3%(78/105)and 61.0%(64/105),respectively.Aberrant expression levels of both p53 gene and telomerase in patients with colorectal cancer were significantly correlated with lymph node metastasis(P<0.05)and their simultaneous measurement could raise the prediction accuracy of metastasis.Expression of p53 gene was also significantly correlated with differentiation degree of colorectal carcinoma(P=0.003).CONCLUSION:Aberrant p53 gene expression and telomerase activity in circulating tumor cells might be malignant biomarkers.Both were closely related and could predict nodal involvement.更多还原