【摘要】 目的:探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中pokemon表达和p14ARF、bcl-2表达的关系及其对NSCLC临床病理特征和预后的判定价值。方法:应用SP免疫组织化学方法检测pokemon和p14ARF、bcl-2三种蛋白在62例NSCLC肿瘤组织及20例癌旁正常组织中的表达,分析它们与NSCLC病理学特征的关系,以及pokemon与p14ARF、bcl-2的相关性,并结合随访资料观察上述蛋白表达对NSCLC长期预后的影响。结果:正常肺组织中未见pokemon蛋白表达,p14ARF和bcl-2蛋白阳性表达率分别为95.0%和15.0%;在NSCLC组织中pokemon、p14ARF和bcl-2阳性表达率分别为72.6%、66.1%和53.2%。Pokemon表达与p14ARF表达呈负相关(r=-0.287,P<0.05),与bcl-2表达呈正相关(r=0.293,P<0.05)。Pokemon和p14ARF的表达与TNM分期相关(P<0.05),bcl-2表达与病理分型相关(P<0.05)。Pokemon与bcl-2表达阳性组的5年生存率分别为10.95%和13.74%,显著低于阴性组(P<0.05);p14ARF表达阳性组的5年生存率为21.68%,显著高于阴性组(P<0.05)。结论:NSCLC组织中存在pokemon和p14ARF、bcl-2的表达,pokemon表达与p14ARF表达呈负相关、与bcl-2表达呈正相关,它们对NSCLC的预后评估有一定的临床意义。更多还原

【Abstract】 Objective:To investigate the correlation of the expression of pokemon with p 14ARF and bcl-2 in non small cell lung cancer (NSCLC) tissues, and their values for determining the clinicopathological characteristics and prognosis of NSCLC. Methods: Immunohistochemical SP staining was used to detect the expressions of pokemon, p 14ARF, and bcl-2 proteins in 62 NSCLC tumor tissues and 20 adjacent normal tissues. Their relationship with the pathological characteristics of NSCLC was analyzed. The correlation of abnormal expressions of pokemon and p 14ARF and bcl-2 was analyzed. The effects of the expressions of the three proteins on the long-term prognosis of NSCLC patients were observed based on the follow-up data. Results:In normal tissues there was no pokemon expression but the positive rates of p 14ARF and bcl-2 expression were 95.0% and 15.0%, respectively. In NSCLC tissues, the positive rate!of pokemon, p 14ARF,and bcl-2 protein were 72.6%, 66.1%, and 53.2%, respectively. The expression of pokemon had negative correlation with expression of p 14ARF (r=-0.287, P<0.05), but had positive correlation with expression of bcl-2 (r=0.293, P<0.05). The expression of pokemon and p 14ARF was associated with TNM staging (P<0.05). However, the expression of bcl-2 correlated with pathological classification (P<0.05). In pokemon and bcl-2 positive patients, the five-year survival rates were 10.95% and 13.74%, respectively, which were significantly lower than that in pokemon and bcl-2 negative patients (P<0.05). In contrast, the five-year survival rate was 21.68% in patients with positive expression of p 14ARF, which was significantly higher than that in patients with negative expression of p 14ARF (P<0.05). Conclusion:Pokemon, p 14ARF, and bcl-2 are all expressed in NSCLC tissues. There is a negative correlation between the expression of pokemon and p 14ARF and a positive correlation between the expression of pokemon and bcl-2, which has a certain clinical significance for evaluating the prognosis of NSCLC patients.更多还原