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毛蚶中抗癌活性肽高效液相指纹图谱分析研究(英文)

Fingerprint Analysis of Anti-tumor Active Polypeptides from Arca subcrenata by HPLC

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【作者】 任胜芳宋丽艳严春艳李婷菲赵昱于荣敏

【Author】 REN Sheng-fang1,SONG Li-yan1,2,YAN Chun-yan1,LI Ting-fei1,ZHAO Yu2,YU Rong-min1(1.College of Pharmacy,Jinan University,Guangzhou 510632,China;2.College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China)

【机构】 暨南大学药学院浙江大学药学院

【摘要】 应用RP-HPLC技术分析毛蚶中的抗癌活性肽。毛蚶活性肽的最优分离条件为:温度:30℃;波长:280nm;流速:1.0 ml/min;流动相A为80%乙腈(含0.1%TFA),流动相B为0.1%TFA水溶液,梯度洗脱。应用上述条件,成功地得到了10批样品的指纹图谱,并确定了14个共有峰。

【Abstract】 RP-HPLC was applied to analyze active polypeptides in Arca subcrenata, and the optimal condition for separation was also set up: temperature: 30℃;wavelength: 280 nm;flow rate: 1.0 ml/min;Solvent A consisted of 80% acetonitrile and 0.1% trifluoroacetic acid(TFA) and solvent B contained 0.1% TFA.In this condition,ten samples′ fingerprints were gained,in one of which the genuine fraction exhibited fourteen "common peaks" representing the characteristics of the constituents.

【基金】 广东省科技计划重大专项(2004A30505003);广州市科技计划重点项目(2005Z2-E4031)
  • 【文献出处】 中药材 ,Journal of Chinese Medicinal Materials , 编辑部邮箱 ,2008年08期
  • 【分类号】R284
  • 【被引频次】8
  • 【下载频次】251
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