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紫花苜蓿逆境胁迫诱导相关转录因子MsDREB1基因克隆与分析
Cloning and Analysis of a New Stress-inducible Transcription Factor MsDREB1 Gene from Alfalfa (Medicago sativa L.)
【摘要】 利用RT-PCR方法,从紫花苜蓿中扩增得到一个新的转录因子MsDREB1基因cDNA,克隆该cDNA并进行序列分析,结果表明该cDNA包含一个长651 bp的开放阅读框,编码一条含216个氨基酸的多肽,分子量约为24.9 kDa,等电点为6.11。蛋白质Blast数据显示,该多肽属于EREBP/AP2家族DNA结合蛋白的典型成员。进一步克隆了该基因的基因组DNA,序列分析表明该基因无内含子。Southern blot分析表明,该基因在紫花苜蓿基因组中以2拷贝存在。
【Abstract】 A new DRE-binding protein gene MsDREB1 cDNA that encoded an EREBP/AP2 type transcription factor was isolated by RT-PCR from alfalfa (Medicago sativa L.) seedlings.The MsDREB1 had an open reading frame of 651 bp,which encoded 216 amino acid residues.The putative protein was deduced to have a predicted molecular mass of 24.9 kDa and a p1 of 6.11.The Protein Blast data revealed that this protein could be classified as a typical member of the EREBP/AP2 family of DNA-binding proteins.The comparison of the MsDREB1 cDNA with the MsDREB1 gene in genomic DNA showed that the size and nucleotide sequence of the cDNA were the same as that of the genomic DNA.This indicates that the genomic MsDREB1 gene has no introns. Southern blot analysis indicates that it is a double-copy gene.
【Key words】 alfalfa (Medicago sativa L.); DREB transcription factor; stress;
- 【文献出处】 植物生理学通讯 ,Plant Physiology Communications , 编辑部邮箱 ,2008年03期
- 【分类号】S541.9;Q943.2
- 【被引频次】8
- 【下载频次】325