【摘要】 采用单侧寡聚核苷酸嵌套PCR(SON-PCR)方法,从长鞭红景天中扩增得到查尔酮合成酶基因(chalcone synthase,CHS),命名为Rhchs,其序列全长为2 443 bp,包括682 bp的启动子区、957 bp的开放阅读框和3′UTR区。预测序列编码381个氨基酸,其氨基酸组成与其他已知的高等植物的查尔酮合成酶具有很高的同源性,与葡萄、山茶花、杜鹃和牵牛的同源性分别为87%、87%、86%和85%。且大部分氨基酸序列保守。序列分析表明,在启动子区域也找到了TATA-box、W-box、G-box like等顺式作用元件。更多还原

【Abstract】 A new complete DNA sequence of chalcone synthase(CHS)gene,which was specially expressed in Rhodiola sachalinensis,was cloned by single oligonucleotide nested PCR(SON-PCR).The gene,named Rhchs, contained a length of 2 443 bp including a promoter region of 682 bp,an ORF of 957 bp and 3′UTR area.CHS protein predicted from Rhchs showed 87%,87%,86%,85% identities with CHS of Vitnus vinifera,Camellia sinensis, Rhododendron simsii,Petuniaxhybrida and retained most of the conserved residues.Some putative cis elements characteristic of the CHS gene such as TATA-box,W-box,G-box were found at the promoter region of Rhchs.更多还原