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大豆茎溃疡病菌TaqMan MGB探针实时荧光PCR检测

Real-time fluorescent PCR detection of Diaporthe phaseolorum var. caulivora and D. phaseolorum var. meridionalis by using TaqMan MGB probe

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【作者】 王颖； 陈枝楠； 赵巍巍； 程颖慧； 章桂明；

【Author】 WANG Ying1, CHEN Zhi-nan1, ZHAO Wei-wei2, CHENG Ying-hui1, ZHANG Gui-ming1 (1Shenzhen Exit-Entry Inspection and Qua-rantine Bureau, Shenzhen 518010, China; 2Hunan Agricultural University, Changsha 410128, China)

【机构】 深圳出入境检验检疫局； 湖南农业大学；

【Abstract】 The technique of TaqMan MGB real-time fluorescent PCR was established to detect Diaporthe phaseolorum var.caulivora(DPC) and D. phaseolorum var.meridionalis (DPM). The primers and TaqMan MGB probes were designed based on the ITS of DPC, DPM, D. phaseolorum var. sojae and Phomopsis longicolla. A series of genomic DNA dilution were used to detect sensitivity of the technique, the results showed that the limits of detection for DPM and DPC were 7 fg/μL and 6 fg/μL DNA respectively.

【关键词】 Diaporthe phaseolorum var.caulivora； D. phaseolorum var. meridionalis； TaqMan MGB； real-time fluorescent PCR；
【Key words】 Diaporthe phaseolorum var.caulivora； D. phaseolorum var. meridionalis； TaqMan MGB； real-time fluorescent PCR；
【基金】 国家质量监督检验检疫总局课题资助项目(2003IK071)
  • 【文献出处】 植物病理学报 ,Acta Phytopathologica Sinica , 编辑部邮箱 ,2008年05期
  • 【分类号】S435.651
  • 【被引频次】4
  • 【下载频次】194
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