【摘要】 为了阐明大豆受尖孢镰刀菌(Fusarium oxysporum,Fo)侵染后在基因转录水平上的感病反应,本研究用cDNA-AFLP方法,对Fo浸根接种后大豆根组织进行了基因转录谱的银染法检测分析。结果表明,在测定的大约1 000个大豆cDNA扩增片段(TDFs)中,16个是差异性表达的,其中9个受Fo诱导上调表达,7个被抑制下调表达。对这些差异性表达的TDFs进行克隆、测序及其同源性进行分析,发现其中6个具有已知或推断的生物学功能,包括钙调素结合蛋白(CaMBP)、BURP结构域蛋白和羟基多花碱-羟基烷宁转移酶等,其余10个功能未知。这些可能参与大豆感病性的差异性表达新基因片段的发现,为开展大豆感病性功能基因组学研究提供了材料。更多还原

【Abstract】 To demonstrate the susceptible response of soybean to Fusarium oxysporum(Fo) at the transcriptional level,gene expression profile in soybean roots during Fo infection was tested using cDNA-AFLP approach.Among approximately 1 000 transcript-derived fragments(TDFs) screened,16 were differentially regulated(9 TDFs induced and 7 repressed) in soybean roots during fungal challenge.Cloning and sequencing of the differentially-expressed TDFs indicated that 6 fragments showed homology to genes with known or putative functions,while 10 others did not show any homology to sequences with known functions.The data from this study confirms the effectiveness of cDNA-AFLP technique in detecting differentially-expressed genes during pathogenesis of soybean.更多还原