【摘要】 目的建立高效液相色谱-紫外-电喷雾质谱(HPLC-UV-ESI/MS)联用测定槟榔食品中槟榔碱的方法。方法以甲醇和0.1%三乙胺水溶液作流动相,在反相C18(5μm,250mm×4.6mm)柱上梯度淋洗,以质谱定性,紫外定量进行检测。结果在0.2～20μg/ml内峰面积和浓度呈良好的线性关系。结论该方法抗干扰能力强,可以快速、准确地测定槟榔食品中的槟榔碱。更多还原

【Abstract】 Objective A method for determining arecoline in betelnut foods by on line HPLC-UV-ESI/MS was established.Methods The separation was operated on an analytical spherigel C18 column(5 μm,250 mm×4.6 mm)with acetonitrile:water(containing 0.1% triethylamine)as mobile phase.Results Good linearity between peak areas and concentrations was obtained within the range of 0.2-20μg/ml.Conclusion The method is simple and can determine arecoline in betelnut foods accurately.更多还原