【摘要】 采用原位杂交技术(In situ hybridization,ISH)研究雄激素受体(Androgen receptor,AR)mRNA在中华鳖(Pelodiseus Sinensis)脾脏的存在及细胞定位。取5只健康成年中华鳖的脾脏,进行石蜡切片。用地高辛标记的寡核苷酸探针对组织切片进行原位杂交(In situ hybridization,ISH),检测雄激素受体mRNA在脾脏免疫细胞内的表达定位。结果显示,大部分雄激素受体mRNA阳性细胞呈圆形或椭圆形,数量少胞体大且呈不规则形。阳性细胞在脾脏动脉周围淋巴鞘(Periaterial lymphatic sheath,PALS)和红髓(Red pulp,RP)内分布密集。椭球周围淋巴鞘(Periellipsoidal lymphatic sheath,PELS)内AR mRNA阳性细胞分布极少。杂交阳性信号物质在细胞质和细胞核均有分布。在标记为阳性的中华鳖脾脏中,推测圆形的阳性细胞可能为B淋巴细胞,而另一部分胞体大且形状不规则的阳性细胞则可能为巨噬细胞。脾脏内AR mRNA的存在进一步证实雄激素可能是调节脾脏等免疫器官功能的因素之一。更多还原

【Abstract】 The reptile’s immune system is strikingly affected by seasonal variation,which induces changes in the structure of the lymphoid organs and in the function of the immune cells. Morphological changes of lymphoid organs (thymus and spleen) throughout the year may relate to annual variations of circulating steroid hormones. Androgen is a kind of steroid hormones and may have direct effects on immune system via androgen receptors. Therefore,to study whether androgen receptor mRNA exists in spleen of reptile and its cellular localization is very important. In this study,Paraffin sections of spleen in five healthy Pelodiseus Sinensis were made. In situ hybridization (ISH) was conducted on the tissue sections by oligonucleotide probe marked by cardiox. The expressions and localization of androgen receptor mRNA were detected in the cells of spleen tissue. The results of in situ hybridization indicate that the AR positive cell distributing in the periaterial lymphatic sheath (PALS) and red pulp were more than those in the periellipsoidal lymphatic sheath (PELS). The results of this study demonstrate that AR mRNA exists in immune cells of spleen. Most AR mRNA positive cells are round or oval- shap in shape,the others are larger and more irregular. The signals distributed in cytoplasm and nuclei of all positive cells. The results suggest that the round or oval-shap cells might be B lymphocytes,the others might be macrophages.The presence of androgen receptor mRNA in the spleen would provide further evidence that androgen might be one of the factors which influence function of spleen and other immune organs.更多还原