【摘要】 目的:探索胶质源性神经营养因子(GDNF)在体外诱导小鼠胚胎中脑神经干细胞(M-NSCs)分化成多巴胺能神经元的培养方法,为M-NSCs移植治疗帕金森病(PD)提供实验依据。方法:在有血清条件下体外培养鼠胚M-NSCs,予以GDNF作诱导分化。TH免疫细胞化学鉴定,流式细胞术检测TH-ir阳性神经元的百分率。结果:M-NSCs向多巴胺能神经元分化的阳性率经流式细胞术检测结果,两组TH-ir阳性细胞比率A组(实验组,6只)为13.53%±1.53%;B组(对照组,6只)3.46%±0.77%,两组比较差异有显著性意义(P<0.01)。结论:GDNF可促进M-NSCs分化成多巴胺能神经元。更多还原

【Abstract】 Objective:To explore the culture methods for the differentiation of mesencephalic neural stem cells(M-NSCs) from embryonic mice into dopaminergic(DAergic)neurons in vitro,and to provide the experimental information for the treatment of Parkinson′s disease by cell transplantation.Method:M-NSCs were cultured and proliferated,then GDNF were used to induce differentiation in the condition of fetal bovine serum (FBS).Immunocytochemical techniques were used to detect the TH-ir cells from NSC differentiation,and FCM to detect the percentage of TH-ir in all the cells. Result:The proportions of M-NSCs differentiated into DAergic neurons were 13.53%±1.53% in GDNF group,and 3.46%±0.77% in control group(P<0.01). Conclusion: GDNF can significantly increase the differentiation of DAergic neurons.更多还原