【摘要】 目的:研究芩连四物汤(川芎、当归、白芍、地黄、黄芩、黄连)中挥发油成分,并探讨其对催产素诱发小鼠离体子宫收缩效应的影响。方法:采用水蒸汽蒸馏法提取芩连四物方及其富含挥发油的当归、川芎组方药材的挥发油,并通过气相色谱-质谱联用技术对其分析鉴定和比较。结果:共鉴定成分70种,考察归一化计算面积大于0.5的29种成分,结果显示复方中的成分除(Z,Z-)9,12-Octadecadienoic acid外,均来源于药材,当归药材中除Cis-ocimene、γ-Cadinene、Aris-tolone成分在复方中未找到,其它均出现在复方中,川芎油中除Aristolone成分外,均出现在复方油中,藁本内酯是复方及组方药材挥发油中的主要成分。结论:对芩连四物方挥发油及其组方药材挥发油进行GC-MS成分比较分析;并提示藁本内酯可能是抑制离体子宫收缩的主要活性成分,为揭示芩连四物汤的效应物质基础提供依据。更多还原

【Abstract】 AIM:To study the components of volatile oil of Qinlian Siwu Decoction(Rhizoma chuanxiong,Radix angelicae sinesis,Radix paeoniae alba,Radix rehmanniae,Radix scutellariae,Rhizoma coptidis,etc) and evaluate the activity of inhibiting the uterine contraction on mice in vitro.METHODS:The chemical compositions of essential oils obtained by steam distillation from Qinlian Siwu Decoction and its major constituting herbs(Radix Angelicae sinensis,Rhizoma chuanxiong),were analyzed by GC-MS.RESULTS:29 components(70 in total) were recognized as the main ones from volatile oils of Qinlia Siwu Decoction.Its comparative peak areas came up to more than 0.5% of total.(Z,Z)-9.12-octadecadienoic acid were not found;and cir-ocimene,γ-cadinene and aristolone from Radix angeliae sinesis;aristolone came from Rhizoma chuanxiong.Ligustilide content was the highest one in volatile oils.CONCLUSION:The chemical components of volatile oil of Qinlian Siwu Decoction and its major constituting herbs(Radix Angelicae sinensis,Rhizoma chuanxiong) are comparatively analysed by GC-MS for the first time.The volatile oil of Qinlian Siwu Decoction shows antagonistic effect on the contraction of the isolated mouse uterus.Ligustilide are possible main active components.更多还原