【摘要】 目的考察青龙衣多糖对S₁₈₀小鼠红细胞内Ca²⁺和H⁺的影响。方法建立肿瘤动物模型,分高、中、低剂量腹腔给予青龙衣多糖7d,采集红细胞,制备红细胞悬液。应用激光扫描共聚焦显微镜结合荧光探针Fluo-3/AM染色观察和测定S₁₈₀小鼠红细胞内Ca²⁺的变化,结合荧光探针BCECF-AM染色观察和测定S₁₈₀小鼠红细胞内H+的变化。结果青龙衣多糖能降低S₁₈₀小鼠红细胞内Ca²⁺,与阴性对照组相比较有显著性差异（P<0.05）;青龙衣多糖各剂量组均能显著降低S₁₈₀小鼠红细胞膜内的H⁺浓度,与阴性对照组相比较有显著性差异（P<0.01）。结论青龙衣多糖组能维持S₁₈₀小鼠红细胞内阳离子浓度稳定平衡。更多还原

【Abstract】 Objective To study the effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S₁₈₀ mice.Methods A tumor mouse model was developed,and the mice were treated with high,medium,and low doses of qinglongyi polysaccharide through abdominal injection for 7d.Erythrocytes were collected and erythrocyte suspensions were prepared.Change of Ca²⁺concentration in the erythrocytes of S₁₈₀mice was measured by stained with Fluo-3/AM and observed with laser scanning confocal microscope,Change of H⁺ concentrations measured by stained with BCECF-AM and observed with laser scanning confocal microscope.Results Qinglongyi polysaccharide lowered the concentration of Ca²⁺and H⁺ in erythrocytes of S₁₈₀mice,which is remarkable compared with negative control group（P<0.05）.Conclusion Qinglongyi polysaccharide can maintain the stability and balance of cation concentration in erythrocyte of S₁₈₀mice.更多还原