【摘要】 目的:观察慢性缺氧所致肺动脉高压时电镜下肺动脉内皮的形态改变、循环内皮细胞(CEC)数量及形态变化、一氧化氮(NO)和酯质过氧化物(LPO)的变化。方法:应用肺动脉微飘浮导管技术、透射电镜技术、细胞分离技术等。结果:缺氧后血管内皮细胞落成为循环内皮细胞。缺氧2 w后,血CEC、NO、MDA升高;缺氧4 w组各值与缺氧2 w组及与正常组比较有显著差异(P<0.01)。结论:缺氧可能通过增加氧自由基、NO释放,加重肺动脉高压血管内皮的损伤、凋亡和脱落。更多还原

【Abstract】 Objective:To investigate the changes of morphology of vascular endothelial cells and the number of circulatory endothelial cells(CEC) in the circulation system,as well as the levels of nitric oxide(NO) and lipid peroxidation(LPO) in rats with hypoxic pulmonary hypertension induced by chronic hypoxia.Methods:Two kinds of hypoxic model of rats were established in decompression chamber for 2 weeks and 4 weeks,respectively.Then their CEC in the circulation blood were collected.The changes of the morphology and the number of CEC were observed under electron microscope and multiple functional microscope and the levels of NO and LPO in CEC were examined.Results:After hypoxia for 2 weeks,the number of CEC and the levels of NO and MDA increased with a significant difference from those of the normal group(P<0.01).After hypoxia for 4 weeks,above changes became very remarkably with a significant difference from those of the normal group and hypoxia 2 weeks group(P<0.01).Conclusion:Hypoxia will result in injury,apoptosis and exfoliation of vascular endothelial cells in rats with pulmonary hypertension by means of promoting releasing of oxygen free radial and NO.更多还原