【摘要】 为研究中药有效成分小檗碱、绿原酸和黄芩苷的抗炎机制,通过体外培养大鼠肠黏膜微血管内皮细胞(Rat intestinal mucosa microvascular endothelial cells,RIMMVECs)建立LPS损伤模型,采用免疫细胞化学方法观察了3种中药成分对LPS损伤RIMMVECs表达ICAM-1的影响,并对染色结果进行了图像灰度分析与统计学检验。研究发现,RIMVECs正常状态下低水平表达ICAM-1,用LPS刺激后ICAM-1的表达明显升高,而中药有效成分小檗碱、绿原酸和黄芩苷能够抑制LPS诱导的RIMVECs强阳性表达ICAM-1。结果表明,小檗碱、绿原酸和黄芩苷的抗炎作用与其下调ICAM-1的表达密切相关,为阐明其抗炎机制提供了数据。更多还原

【Abstract】 To investigate their anti-inflammatory mechanism,the effects of berberine(Ber.),chlorogenic acid(Ch.A.) and baicalin(Bai) on the expression of intercellular adhesion molecule 1(ICAM-1) in rat intestinal mucosa microvascular endothelial cells(RIMMVECs) challeged by lipopolysaccharide(LPS) were analyzed by immunocytochemical staining,and gray scale and statistical analysis were performed.The results showed that RIMMVECs constitutively express ICAM-1 in low level and LPS significantly increased it,while LPS-induced strong expression of ICAM-1 in RIMMVECs was suppressed by Ber,Ch.A and Bai.The results indicated that the anti-inflammatory role of Ber,Ch.A and Bai is related to their downregulating effect on LPS-mediated ICAM-1 expression, which provide data for clarifying their mechanism.更多还原