【摘要】 筛选了人肺腺癌细胞A549放射前后的差异蛋白质,并探讨了其在放射中的作用。用双向凝胶电泳对人肺腺癌细胞A549放射前后的蛋白质谱进行分析,寻找差异蛋白质,并用MALDI-TOF-MS进行蛋白质鉴定。检测出25个差异蛋白质点,对其中差异2倍以上的7个蛋白质点用MALDI-TOF-MS鉴定,鉴定出锰超氧化物歧化酶B亚型前体、角蛋白8、角蛋白9、dystrobrevin、不均一核糖核蛋白1(HNRPH1)、烯醇化酶1等6种蛋白质。用双向凝胶电泳筛选细胞系放射前后差异蛋白质,筛选的蛋白质与抗氧化、辐射防护、信号转导、RNA加工和转运、能量代谢及细胞凋亡有关,对于揭示放射的分子机制有一定的意义。更多还原

【Abstract】 To screen differential expression protein of human lung adenocarcinoma cell A549 treated with radiation and to discuss the role of these proteins. Differential expression protein in A549 cell treated with radiation were screened by 2D-PAGE and identified by MALDI-TOF-MS. Twenty-five differential expression protein spots were found in A549 cell treated with radiation by 2D-PAGE, and the six proteins (manganese superoxide dismutase isoform B precursor, keratin 9, dystrobrevin, HNRPH1, enolase 1, keratin 8) were identified by MALDI-TOF-MS. The differential expression protein between the treated with radiation by 2D-PAGE relate to antioxidation, radioprotection, signal transmission, RNA processing and transfering, cell apoptosis. It is possibly significance for explaining molecule mechanism of the responds of cell to radiation.更多还原