【摘要】 以小鼠为试验动物,分别经腹腔注射产志贺样毒素大肠埃希菌菌液和志贺样毒素二型变异体粗制品,复制猪水肿病病理模型。结果表明,小鼠最佳感染细菌数为1.6×1010,志贺样毒素二型变异体最佳感染剂量为0.25 mg。大肠埃希菌菌菌液和志贺样毒素二型变异体人工感染小鼠,其临床症状、病理变化没有明显差异。主要病理组织学变化为:肝变性、坏死,脾巨细胞增生、部分淋巴细胞坏死,肺充血,肾间质血管微血栓形成,小肠绒毛结构被破坏,固有层水肿,间质微血管充血,大脑噬神经现象和卫星化现象等。结果表明志贺样毒素二型变异体是引起猪水肿病主要致病因子,小鼠可作为研究猪水肿病的动物模型。更多还原

【Abstract】 The Shiga-like toxin Escherichia coli and shiga like toxin Ⅱ variant(SLT-Ⅱe) that can establish pathology model of swine edema disease was injected to mouse’s abdominal cavity.The results showed that the prime number of bacteria and SLT-Ⅱe is 1.6×1010and 0.25mg.The clinic symptom and pathology observe wansn’t evidence difference.The main pathology histology change included liver denaturalization,necrosis,spleen giant cell hyperplasia,lymphocyte necrosis,lung and kidney microthrombus form,small intestine edema,microvascular hyperemia,cerebrum neuronophagia and satellitosis.The results showed that SLT-Ⅱe is the main nosogenesis of swine edema disease and mouse can become animal model of swine edema disease.更多还原