【摘要】 用琼脂糖(agrose)将肌红蛋白(Mb)固定在玻碳电极(GCE)表面,制备了Mb-Agrose膜修饰电极。在乙醇亲水性有机溶剂与水的混合溶液中,包埋在Agrose中的Mb可与电极发生直接电子传递,且能催化还原过氧化氢、氢过氧化异丙基苯、氢过氧化叔丁基异丙苯、过氧化丁酮等过氧化物。Mb-Agrose膜修饰电极可用于上述过氧化物的定量检测。更多还原

【Abstract】 Myoglobin(Mb) was immobilized on glassy carbon electrode(GCE)surface by agrose film.Mb entrapped in the agrose film underwent fast direct transfer-electron reactions in water-ethanol solvent mixtures.The electrocatalysis of peroxides,such as hydrogen peroxide,cumene hydroperoxide,cumene t-butyl hydroperoxide,and 2-butanone peroxide,by the immobilized Mb was also explored.The good stability and reproducibility of the modified electrode made it possible to determine the organic peroxides successfully.更多还原