【摘要】 目的:通过卵巢癌HO-8910细胞表面的TfR(CD71)在rhEPO干预前后的表达情况来观察rhEPO对细胞增殖的影响,探讨其在肿瘤相关性贫血中的治疗价值。方法:用RT-PCR法检测经不同浓度的rhEPO处理后的和未经rhEPO处理的HO-8910细胞表面的TfR mRNA表达情况:用流式细胞仪观察和检测处理前后的HO-8910细胞周期上的差异。结果:不同浓度的rhEPO培养HO-8910细胞48 h,72 h后,TfR表达均减少,与同期对照组相比差异有显著意义(P<0.01);经不同浓度的EPO在不同时间处理后的HO-8910及未经EPO处理的HO-8910在细胞周期上,与同期对照组相比差异有显著意义(P<0.01),且较对照组有所减少。结论:经过高浓度的rhEPO处理后,卵巢癌HO-8910细胞表面TfR的表达减少,S期细胞百分率也有所降低,间接提示了高浓度的rhEPO可能通过铁代谢抑制卵巢癌HO-8910细胞的生长、增殖。更多还原

【Abstract】 Objective:To detect the expression of TfR residing on the surface of the HO-8910 cultured with EPO and cultrured without EPO,accordingly to observe the effect of EPO on the proliferation of human cancer cell line HO-8910,and approach the mangagement value of EPO.Methods:To detect the expression of TfR residing on the surface of the HO-8910 cultured with EPO and without EPO by RT-RCR.To detect the difference of cancer cell cycle by FCM.Results:The expression of TfR of the HO-8910 cultured with EPO decreased to that of the HO-8910 cultured without EPO,and it had statistical singificance.The percentage of the S cell cycle of the HO-8910 cultured with EPO did not decrease to that of the HO-8910 cultured without EPO.Conclusion:The high concentration of EPO might repress the HO-8910 proliferation.更多还原