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激活素A对小鼠巨噬细胞RAW264.7吞噬活性的促进作用

Activin A Enhances Phagocytosis of Murine Macrophage Cell Line RAW264.7

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【作者】 霍德胜柳忠辉王世瑶台桂香冯野张宸豪张鹏宇刘海岩

【Author】 HUO De-sheng, LIU Zhong-hui, WANG Shi-yao, et al (Department of Immunology, Norman Bethune College of Medical Sciences, Jilin University, Changchun 130021, China)

【机构】 吉林大学白求恩医学院免疫学教研室吉林省神经免疫重点实验室吉林大学中日联谊医院外科

【摘要】 目的探讨激活素A对巨噬细胞吞噬活性的促进作用及其机制。方法分别通过中性红试验、鸡红细胞法和流式细胞术,检测激活素A对巨噬细胞系RAW264.7细胞的吞饮活性、吞噬活性以及对MHCⅠ、Ⅱ类分子和CD68表达的影响。结果激活素A可明显促进RAW264.7细胞的吞饮及吞噬活性,对MHCI、II类分子表达没有影响,但可明显上调巨噬细胞表面分子CD68的表达。结论激活素A可促进巨噬细胞系RAW264.7细胞吞噬活性,这与其促进巨噬细胞成熟有关。

【Abstract】 Objective To explore the mechanism of activin A in enhancing the phagocytosis of macrophages. Methods Determine the murine macrophage cell line RAW264.7 treated with activin A for pinocytosis by neutral red test, for phagocytosis by chicken red blood cell test, and for expressions of MHC Ⅰand Ⅱ as well as CD68 by flow cytometry. Results Activin A enhanced both the pinocytosis and phagocytosis of RAW264.7 cells significantly, while showed no significant influence on the expressions of MHCⅠand Ⅱ. However, the expression of CD68 was upregulated significantly. Conclusion Activin A enhanced the phagocytosis of macrophage cell line RAW264.7, which was related to its promoting effect on maturation of macrophages.

【关键词】 巨噬细胞RAW264.7激活素A吞噬
【Key words】 MacrophageRAW264.7Activin APhagocytosis
【基金】 国家自然科学基金(30571688、30671953);吉林省科技厅项目(2004070726)
  • 【文献出处】 中国生物制品学杂志 ,Chinese Journal of Biologicals , 编辑部邮箱 ,2008年09期
  • 【分类号】R392.12
  • 【被引频次】22
  • 【下载频次】631
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